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aav8 esyn dio enphr3 0 egfp  (Vector Biolabs)


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    Structured Review

    Vector Biolabs aav8 esyn dio enphr3 0 egfp
    a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received <t>AAV8-Syn-DIO-eNpHR3.0-EGFP</t> virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.
    Aav8 Esyn Dio Enphr3 0 Egfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav8+esyn+dio+enphr3+0+egfp/AAV-eSYN-DIO-eNpHR3%2E0-mCitrine/bio_rxiv__2020__07__02__183780-54-24-25
    Average 95 stars, based on 2 article reviews
    aav8 esyn dio enphr3 0 egfp - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Activation of prodynorphin neurons in the dorsomedial hypothalamus inhibits food intake and promotes positive valence"

    Article Title: Activation of prodynorphin neurons in the dorsomedial hypothalamus inhibits food intake and promotes positive valence

    Journal: bioRxiv

    doi: 10.1101/2020.07.02.183780

    a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received AAV8-Syn-DIO-eNpHR3.0-EGFP virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.
    Figure Legend Snippet: a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received AAV8-Syn-DIO-eNpHR3.0-EGFP virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.

    Techniques Used: Plasmid Preparation, Construct, Injection, Expressing

    Related Articles

    Mouse Assay:

    Article Title: Activation of prodynorphin neurons in the dorsomedial hypothalamus inhibits food intake and promotes positive valence
    Article Snippet: Arg3.1-Cre/ER T2 mice (12–20-week-old) were anesthetized with a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg), and positioned on a stereotaxic instrument (Narishige, Tokyo, Japan). .. Mice were injected in each side or one side of the DMH with ∼0.5 μL AAV2-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, Cambridge, USA), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (Addgene), AAV8-eSyn-DIO-EGFP (Addgene) or AAV8-eSyn-DIO-eNpHR3.0-EGFP (Vector Biolabs, Malvern, PA, USA) using the following coordinates: AP: −1.85 mm, L: ± 0.3 mm, DV: −5.5 mm. ..

    Injection:

    Article Title: Activation of prodynorphin neurons in the dorsomedial hypothalamus inhibits food intake and promotes positive valence
    Article Snippet: Arg3.1-Cre/ER T2 mice (12–20-week-old) were anesthetized with a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg), and positioned on a stereotaxic instrument (Narishige, Tokyo, Japan). .. Mice were injected in each side or one side of the DMH with ∼0.5 μL AAV2-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, Cambridge, USA), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (Addgene), AAV8-eSyn-DIO-EGFP (Addgene) or AAV8-eSyn-DIO-eNpHR3.0-EGFP (Vector Biolabs, Malvern, PA, USA) using the following coordinates: AP: −1.85 mm, L: ± 0.3 mm, DV: −5.5 mm. ..



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    Vector Biolabs aav8 esyn dio enphr3 0 egfp
    a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received <t>AAV8-Syn-DIO-eNpHR3.0-EGFP</t> virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.
    Aav8 Esyn Dio Enphr3 0 Egfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav8+esyn+dio+enphr3+0+egfp/AAV-eSYN-DIO-eNpHR3%2E0-mCitrine/bio_rxiv__2020__07__02__183780-54-24-25
    Average 95 stars, based on 1 article reviews
    aav8 esyn dio enphr3 0 egfp - by Bioz Stars, 2026-10
    95/100 stars
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    a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received AAV8-Syn-DIO-eNpHR3.0-EGFP virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.

    Journal: bioRxiv

    Article Title: Activation of prodynorphin neurons in the dorsomedial hypothalamus inhibits food intake and promotes positive valence

    doi: 10.1101/2020.07.02.183780

    Figure Lengend Snippet: a The AAV vector construct used for this experiment. Male Arg3.1-CreER T2 mice received AAV8-Syn-DIO-eNpHR3.0-EGFP virus injection into the DMH. After recovery, the mice were fasted overnight and re-fed RCD. 1 h after refeeding, mice were given 4-OHT injection (i.p.) to induce Cre recombination ( n = 3). b eNpHR3.0 (green) expression in the DMH (injection site). c eNpHR3.0 (green) expression in the PVH. d No expression of eNpHR3.0 (green) in the ARC.

    Article Snippet: Mice were injected in each side or one side of the DMH with ∼0.5 µL AAV2-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, Cambridge, USA), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (Addgene), AAV8-eSyn-DIO-EGFP (Addgene) or AAV8-eSyn-DIO-eNpHR3.0-EGFP (Vector Biolabs, Malvern, PA, USA) using the following coordinates: AP: −1.85 mm, L: ± 0.3 mm, DV: −5.5 mm.

    Techniques: Plasmid Preparation, Construct, Injection, Expressing